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EC Number
kcat/KM Value [1/mMs
-1
]
kcat/KM Value Maximum [1/mMs
-1
]
Substrate
Commentary
Reference
3.3.2.6
1.497
-
L-alanine-7-amido-4-methylcoumarin
in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C
731116
3.3.2.6
1.7
-
L-alanine-4-nitroanilide
wild type enzyme, at pH 7.8 and 22°C
731360
3.3.2.6
3.96
-
L-alanine-4-nitroanilide
mutant enzyme F375Y, at pH 7.8 and 22°C
731360
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