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EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Commentary	Reference
2.2.1.6	0.23	-	pyruvate	mutant F109M, pH 7.6, 37°C	718875
2.2.1.6	0.32	-	pyruvate	wild type enzyme, at pH 7.5 and 20°C	758092
2.2.1.6	0.352	-	pyruvate	pH 7.5, 37°c, recombinant mutant P126V	733745
2.2.1.6	0.375	-	pyruvate	mutant E47A, pH 7.6, 37°C	718895
2.2.1.6	0.46	-	pyruvate	mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C	758092
2.2.1.6	0.46	-	pyruvate	mutant V391A, pH 7.6, 37°C	719466
2.2.1.6	0.52	-	pyruvate	mutant enzyme S627N, at pH 7.5 and 20°C	758092
2.2.1.6	0.7	-	pyruvate	mutant V375A, pH 7.6, 37°C	718875
2.2.1.6	1.2	-	pyruvate	mutant V477I, pH 7.6, 37°C	719466
2.2.1.6	1.3	-	2-oxobutanoate	mutant V375A, pH 7.6, 37°C	718875

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Release 2023.1 (January 2023)