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EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Reference
0.13
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.254
-
oxaloacetate
L87N variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.53
-
oxaloacetate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
2.2
-
oxaloacetate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
10.4
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
12.6
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
44.7
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
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