EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.1.3.43 | 10.4 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 10.4 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
4.1.3.43 | 10.6 |
- |
4-hydroxy-2-oxohexanoate |
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
4.1.3.43 | 11 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 12.6 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 12.6 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
4.1.3.43 | 13 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 13.4 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
4.1.3.43 | 13.5 |
- |
4-hydroxy-2-oxohexanoate |
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
4.1.3.43 | 14.1 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |