EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.1.3.43 | 44.7 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
4.1.3.43 | 0.8 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 19 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 1.6 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 13 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 11 |
- |
4-hydroxy-2-oxopentanoate |
with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C |
721659 |
4.1.3.43 | 0.0015 |
- |
Butyraldehyde |
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 0.0017 |
- |
pentaldehyde |
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 0.002 |
- |
propionaldehyde |
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 0.0025 |
- |
acetaldehyde |
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |