EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.1.3.43 | -999 |
- |
more |
highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides |
721615 |
4.1.3.43 | -999 |
- |
more |
value not detectable for R16A variant of BphI |
714244 |
4.1.3.43 | 0.00001 |
- |
hexaldehyde |
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C |
714244 |
4.1.3.43 | 0.00001 |
- |
hexaldehyde |
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C |
714244 |
4.1.3.43 | 0.00004 |
- |
pentaldehyde |
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 0.000051 |
- |
pentaldehyde |
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 0.0001 |
- |
hexaldehyde |
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C |
714244 |
4.1.3.43 | 0.00013 |
- |
hexaldehyde |
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C |
714244 |
4.1.3.43 | 0.00016 |
- |
Butyraldehyde |
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
4.1.3.43 | 0.00031 |
- |
Butyraldehyde |
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |