EC Number   |
General Information |
Reference |
|---|
   7.1.3.1 | malfunction |
defects in PPase activity cause severe developmental defects and/or growth arrest in several organisms. The fugu5 mutant phenotype, caused by a defect in H+-PPase activity, shows a postgerminative growth phenotype, but is rescued by complementation with the yeast cytosolic PPase IPP1, overview. Increased cytosolic PPi levels Impaired postgerminative development in fugu5 by inhibiting gluconeogenesis |
720775 |
| 7.1.3.1 | malfunction |
the fugu5 mutant is defective in AVP1, i.e. vacuolar H+-pyrophosphatase, due to point mutations A709T, A553T, or E272K, and fails to support heterotrophic growth after germination. Exogenous supplementation of succinate or the specific removal of the cytosolic diphosphate by the heterologous expression of the cytosolic inorganic diphosphatase1, IPP1, gene from Saccharomyces cerevisiae rescues fugu5 phenotypes. Compared with the wild-type and AVP1Pro:IPP1 transgenic lines, hypocotyl elongation in the fugu5 mutant is severely compromised in the dark but recovers upon exogenous supply of succinate to the growth media. The peroxisomal beta-oxidation activity, dry seed contents of storage lipids, and their mobilization are unaffected in fugu5 |
720685 |
| 7.1.3.1 | metabolism |
implication of H+-PPase in postgerminative oilseed metabolism, overview |
720775 |
| 7.1.3.1 | more |
vacuolar proteases are involved in the processing of native AVP1 and its chimaeric derivatives targeted to the vacuolar membrane |
718788 |
| 7.1.3.1 | physiological function |
a co-ordinated action of the enzyme with H+-ATPase at the tonoplast can allow a higher transport capacity at the vacuolar membrane when plants perform high crassulacean acid metabolism |
747230 |
| 7.1.3.1 | physiological function |
co-expression of the enzyme and a vacuolar Na+/H+ antiporter gene from Pennisetum glaucum confers enhanced salt tolerance to the transformed tomato compared with the single gene transgenic plants and the wild type. Co-expression of the enzymes improves the osmoregulatory capacity of double transgenic lines by enhanced sequestration of ions into the vacuole by increasing the availability of protons and thus alleviating the toxic effect of Na+ |
748320 |
| 7.1.3.1 | physiological function |
compared with wild type plants, transgenic alfalfa plants, co-expressing the enzyme and tonoplast cation/H+ antiporter, grow better with greater plant height and dry mass under normal or stress conditions (NaCl or water-deficit) in the greenhouse. Furthermore, the transgenic alfalfa co-expressing both enzymes also grow faster than wild type plants under field conditions and exhibit enhanced photosynthesis capacity by maintaining higher net photosynthetic rate, stomatal conductance, and water-use efficiency than wild type plants |
748903 |
| 7.1.3.1 | physiological function |
enzyme overexpression confers enhanced tolerance to abiotic stresses, including heat shock and H2O2, as well as NaCl, Cd, Mn, Zn, Ca, and Al. Enzyme overexpression results in hypersensitivity to menadione and cobalt |
748051 |
| 7.1.3.1 | physiological function |
enzyme overexpression in transgenic finger millet enhances the plant's performance under salt stress |
748479 |
| 7.1.3.1 | physiological function |
enzyme overexpression leads to higher vacuolar proton currents and vacuolar acidification. After 3 d in salt-untreated conditions, enzyme-overexpressing leaves show a drop in photosynthetic capacity, plasma membrane depolarization and eventual leaf necrosis. Salt, however, rescues enzyme-hyperactive cells from cell death. Under normal growth conditions, plants need to regulate the enzyme activity to avoid hyperactivity and its negative feedback on cell viability |
748775 |
