EC Number   |
General Information |
Reference |
|---|
    6.3.1.9 | malfunction |
genetic knockout mutants of both alleles are not viable, chemical targeting of LiTRYS with a drug-like compound FS-554 also leads to parasite death |
-, 744964 |
| 6.3.1.9 | metabolism |
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase |
-, 746173 |
| 6.3.1.9 | metabolism |
trypanothione synthetase (TryS) of thiol metabolic pathway is the sole enzyme responsible for the biosynthesis of trypanothione in Leishmania donovani |
-, 746270 |
| 6.3.1.9 | metabolism |
trypanothione synthetase catalyses the two-step biosynthesis of trypanothione from spermidine and glutathione |
728592 |
| 6.3.1.9 | more |
binding sites for glutathione, ATP and two Mg2+ ions, molecular docking and molecular dynamics simulations, strructure modeling, detailed overview |
728592 |
| 6.3.1.9 | more |
docking study and structure homology modeling using Leishmania major TryS, PDB ID 2VOB, structure as template, overview |
-, 744910 |
| 6.3.1.9 | more |
the steady-state kinetic analysis discard a ping-pong mechanism |
-, 727951 |
| 6.3.1.9 | physiological function |
culture of a tetracyclinedependent conditional double knockout cDKO parasites without tetracycline induction results in loss of trypanothione and accumulation of glutathione, followed by growth inhibition and cell lysis after 6 days. In the absence of inducer, cDKO cells are unable to infect mice, confirming that the enzyme is essential for virulence in vivo as well as in vitro |
705789 |
| 6.3.1.9 | physiological function |
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis |
-, 746173 |
| 6.3.1.9 | physiological function |
the enzyme is involved in Amp B resistance and during oxidative stress to help the parasites survival |
-, 746270 |
