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EC Number General Information Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.28malfunction Rib2/Pus8p acts on cytoplasmic tRNAs, whereas Pus9p modifies the same position in mitochondrial tRNAs. The DELTArib2/pus8/DELTApus9, is viable only in the presence of 15 mg/liter riboflavin in the growth medium 712356
Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.28malfunction the rluA deletion mutant fails to form either 23S RNA pseudouridine746 or tRNA pseudouridine32. Replacement of rluA in trans on a rescue plasmid restores both pseudouridines. There is no difference in exponential growth rate between wild-type and MG1655(rluA-) either in rich or minimal medium at 24, 37, or 42°C, but when both strains are grown together, a strong selection against the deletion strain is observed 704391
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