EC Number   |
General Information |
Reference |
|---|
    5.1.3.3 | evolution |
enzyme NbAELP belongs to the aldose 1-epimerases, classified in the family of mutarotases |
747879 |
| 5.1.3.3 | evolution |
the galM pseudogene has not been deleted during the evolution of Yersinia pestis to become a vector-transmitted systemic pathogen because its reactivation may be beneficial in certain environments. The galactose mutarotase (galM) gene of Yersinia pestis can be converted to a pseudogene by a single-base-pair deletion, overview |
-, 728233 |
| 5.1.3.3 | malfunction |
knockdown of enzyme NbAELP results in high activity of pectin methylesterase (PME) in the cell wall and a decrease in the leaf glucose level, creating unfavorable conditions for Agrobacterium tumefaciens reproduction in injected leaves. NbAELP gene knockdown followed by NbPME overexpression leads to dwarfism and leaf structure modifications |
747879 |
| 5.1.3.3 | metabolism |
GalM function and regulation in the galactose network, overview |
-, 728233 |
| 5.1.3.3 | metabolism |
the mechanical damage that often precedes the penetration of a leaf by a pathogen promotes the activation of pectin methylesterase (PME). The activation of PME leads to the emission of methanol, resulting in a priming effect on intact leaves, which is accompanied by an increased sensitivity to Tobacco mosaic virus (TMV) and resistance to bacteria, e.g. by induction of enzyme NbAELP |
747879 |
| 5.1.3.3 | physiological function |
methanol-inducible NbAELP is capable of binding the Tobacco mosaic virus (TMV) movement protein (MPTMV) in vitro and is likely to affect the cellular nucleocytoplasmic transport, which may explain the sensitivity of NbAELP knockdown plants to TMV. Although NbAELP is primarily detected in the cell wall, the influence of this protein on cellular PME mRNA levels might be associated with reduced transcriptional activity of the pectin methylesterase (PME) gene in the nucleus. Plant wounding and/or pathogen attack lead to PME activation and increased methanol emission, followed by increased NbAELP expression, which results in reversion of PME mRNA level and methanol emission to levels found in the intact plant. NbAELP downregulates mRNA accumulation directed by PME transcription promoter and downregulates the synthesis of PME mRNA by the feedback principle |
747879 |
| 5.1.3.3 | physiological function |
the two anomers of D-galactose are used for different purposes, alpha-D-galactose as a carbon source and beta-D-galactose for induction of UDP-galactose synthesis for biosynthetic glycosylation |
-, 728233 |
