EC Number   |
General Information |
Reference |
|---|
    4.2.3.29 | evolution |
enzymes KSLs belong to the type A/class I terpene synthase family |
746981 |
| 4.2.3.29 | evolution |
plants have extensively diversified their arsenal of labdane-related diterpenoids (LRDs), in part via gene duplication and neo-functionalization of the ancestral ent-kaurene synthase (KS) required for gibberellin metabolism. Ricinus communis contains 4 RcKSL isozymes, molecular phylogenetic analysis indicates that RcKS(L)1 is significantly more closely related to dicotyl ent-kaurene synthases (KSs), while the other three, RcKSL2-4, cluster separately. RcKSL2-4 are in close proximity to each other, within a region of 65 kb, with RcKSL2 and 4 occurring as a tandem gene pair. RcKS(L)1 is the only isozyme to react with ent-CPP producing small amounts of ent-kaurene, and is referred to as RcKS1. Both RcKSL2 and RcKSL3 also are selectively reacting with ent-CPP with RcKSL2 producing primarily ent-trachylobane (70%) as well as smaller amounts of ent-kaurene (30%), and RcKSL3 producing ent-sandaracopimaradiene (94%) (EC 4.2.3.29) along with small amounts of ent-labdatriene (3%) and ent-pimaradiene (3%). RcKSL4 seems to be inactive, with no products evident from any substrate, but upon synthesis of a codon-optimized gene for the corrected aa sequence and functional analysis, RcKSL4 selectively reacts with ent-CPP and produces largely ent-beyerene (95%) along with very small amounts of ent-atiserene (4%) and ent-kaurene (1%). The enzymes belong to the terpene synthase family and is a class I diterpene synthase |
748876 |
| 4.2.3.29 | metabolism |
LC-MS/MS analyses show that oryzalexins A and C are not detected in either accession W0106 or W1943. Oryzalexins E and F, which are not detected in the two Or accessions, are also undetectable in Oryza sativa cv. Nipponbare. OrKSL10 and OrKSL10ind cannot usually produce ent-sandaracopimaradiene strongly suggesting that no accumulation of oryzalexins A-F is possible in either of the two Or accessions |
746981 |
| 4.2.3.29 | more |
recombinant isozymes OrKSL10j (from cv. W106) and OrKSL10ind (from cv. W1943) have similar activity |
746981 |
| 4.2.3.29 | physiological function |
OrKSL10j and OrKSL10ind encode ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F |
746981 |
| 4.2.3.29 | physiological function |
OsKSL10 encodes ent-sandaracopimaradiene synthase responsible for the biosynthesis of oryzalexins A-F, but oryzalexins E and F are undetectable in Oryza sativa cv. Nipponbare, they are also undetectable in Oryza rufipogon accessions W106 and W1943 |
746981 |
