EC Number   |
General Information |
Reference |
|---|
   4.2.3.152 | malfunction |
in-frame complete deletion of the 2-epi-5-epi-valiolone synthase gene has little effect on in vivo production of shinorine. Complete segregation of the 2-epi-5-epi-Valiolone synthase gene deletion mutant proves difficult and is achieved only when the mutant is grown in the dark and in a medium supplemented with fructose. The segregated mutant shows a striking colour change from native bluegreen to pale yellow-green, corresponding to substantial loss of the photosynthetic pigment phycocyanin, as evinced by combinations of absorbance and emission spectra. Transcriptional analysis of the mutant grown in the presence of fructose under dark or light conditions reveals downregulation of the cpcA gene that encodes the alpha subunit of phycocyanin, whereas the gene encoding nblA, a protease chaperone essential for phycobilisome degradation, is not expressed |
730552 |
| 4.2.3.152 | metabolism |
2-epi-5-epi-valiolone synthase activity is essential for maintaining phycobilisome composition in the cyanobacterium Anabaena variabilis ATCC 29413 when grown in the presence of a carbon source |
730552 |
| 4.2.3.152 | physiological function |
the enzyme is involved in biosynthesis of 2-epi-5-epi-valiolone a precursor in the biosynthesis of C7N-aminocyclitol-containing natural products, such as validamycin and acarbose |
-, 729052 |
| 4.2.3.152 | physiological function |
the product 2-epi-5-epi-valiolone is a precursor in the biosynthesis of the cetoniacytones |
-, 729598 |
