EC Number   |
General Information |
Reference |
|---|
  4.2.3.128 | evolution |
the enzyme belongs to the sesquiterpene synthases |
713839 |
| 4.2.3.128 | evolution |
the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence |
743000 |
| 4.2.3.128 | evolution |
the terpene synthase Mg25 belongs to the angiosperm sesquiterpene synthase subclass TPS-a. The structural diversity observed in the three Magnolia terpene synthase genes have occurred either by a rapid loss of introns from a common ancestor TPS gene or by a gain of introns into an intron-deficient progenote TPS gene |
720729 |
| 4.2.3.128 | metabolism |
the enzyme is part of the biosynthesis of volatile organic compounds in flowers |
743000 |
| 4.2.3.128 | metabolism |
the farnesyl cation can undergo either 1,10- or 1,11-cyclization, giving rise to either an (E,E)-germacradienyl cation or an (E,E)-humulyl cation, respectively |
749042 |
| 4.2.3.128 | more |
directed mutations of the H-alpha1 loop have a marked effect on the product profile Cop4, loop mutations in Cop4 also implicate specific residues responsible for the pH sensitivity of the enzyme. In vivo analysis of sesquiterpene product profiles of H-alpha1 loop mutants, overview. Mutation of K233, presumed to interact with the second Asp92 in the DDXXD motif of Cop4, does not significantly change the overall product promiscuity of Cop4, though beta-cubebene, with 27% of total sesquiterpene products, does become the major product |
713839 |
| 4.2.3.128 | more |
structural modeling, structure-function relationship, overview. Changing the pH of the reaction drastically alters the fidelity of Cop4 and makes it a highly selective enzyme |
714733 |
| 4.2.3.128 | more |
two terpenes dominate the plant sesquiterpene profile, with beta-cubebene accounting for 31.3% of the total and beta-elemene for 25.3%. The enzyme contains the conserved Asp-rich domain, DDXXD, that coordinates substrate binding via the formation of divalent cation salt bridges |
720729 |
| 4.2.3.128 | physiological function |
in sweet orange tissues sesquiterpene profile is qualitatively rich. Overall, up to 25 sesquiterpenes are identified in total. Enzyme CsSesquiTPS1 catalyzes formation of a 62:38% mixture of (Z)-beta-cubebene and alpha-copaene |
747877 |
| 4.2.3.128 | physiological function |
recombinant CoTPS2 catalysed the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene |
743000 |
