EC Number   |
General Information |
Reference |
|---|
   3.4.22.31 | evolution |
despite a high degree of sequence identity between ananain and stem bromelain, the most abundant bromelain cysteine protease, ananain displays distinct chemical properties, substrate preference and inhibitory profile compared to stem bromelain. Ananain belongs to the papain family of peptidases with a high degree of identity to chymopapain (52%) and papain (60%) from papaya |
752864 |
| 3.4.22.31 | more |
the enzyme shows a geometrically flat and open S1 subsite for ananain. This subsite accommodates diverse P1 substrate residues, while a narrow and deep hydrophobic pocket-like S2 subsite would accommodate a non-polar P2 residue, such as the preferred Leu residue observed in specificity studies |
752864 |
| 3.4.22.31 | physiological function |
ananain accounts for less than 10% of the total enzyme in the crude pineapple stem extract known as bromelain, yet yields the majority of the proteolytic activity of bromelain |
752864 |
