EC Number   |
General Information |
Reference |
|---|
  2.8.1.B3 | malfunction |
Bacillus subtilis strains lacking thiI display a specific disruption in the C-4 thiolation of the uridine base at position 8 of tRNA synthesis |
-, 725275 |
| 2.8.1.B3 | malfunction |
deletion of ThiI (MMP1354) abolishes the biosynthesis of 4-thiouridine but not of thiamine |
725494 |
| 2.8.1.B3 | malfunction |
thiI mutants are auxotrophic for thiamine, specifically for the thiazole component of this essential vitamin |
725261 |
| 2.8.1.B3 | metabolism |
the enzyme is crucial for the formation of C-4 thiolation of the uridine base at position 8 of tRNA modification in tRNA |
-, 725275 |
| 2.8.1.B3 | metabolism |
ThiI is a bifunctional enzyme required for the synthesis of both the 4-thiouridine modification in tRNA and the thiazole moiety of thiamine. In 4-thiouridine biosynthesis, ThiI adenylates the tRNA uridine and transfers sulfur from a persulfide formed on the protein. The C-terminal rhodanese domain of ThiI is sufficient for thiazole synthesis in vivo |
725262 |
