EC Number   |
General Information |
Reference |
|---|
  2.7.4.34 | metabolism |
enzyme uses inorganic polyphosphate as a donor to convert GDP to GTP. Polyphosphate chains of 30-50 residues are optimal, those of 15-700 residues can also serve. The rate of polyphosphate utilization is nearly 100fold greater than that of polyphosphate synthesis |
-, 762302 |
| 2.7.4.34 | physiological function |
a PPK2 mutant strain shows significantly increased sensitivity to a range of antibiotics in a manner independent of the mode of action of the antibiotic |
760742 |
| 2.7.4.34 | physiological function |
PPK2 contributes to Mycobacterium tuberculosis ability to cause disease in guinea pigs. PPK2 mutant-infected guinea pigs have significantly reduced bacterial loads and tissue pathology in comparison to wild-type-infected guinea pigs at later stages of infection. In comparison to the wild-type strain, the PPK2 mutant strain is more tolerant to isoniazid and impaired for survival in THP-1 macrophages |
-, 762413 |
| 2.7.4.34 | physiological function |
Ppk2 is required for control of intrabacillary polyphosphate levels and optimal Mycobacterium tuberculosis growth and survival in macrophages and mouse lungs. A Ppk2 insertion mutant has significantly increased polyphosphate content and a 4fold increase in the MIC of isoniazid relative to the wild-type. The mutant shows reduced survival at day 7 in activated and naive J774 macrophages relative to the wild-type. Naive mutant-infected macrophages show increased expression of interleukins IL-2, IL-9, IL-10, IL-12p70, and gamma interferon relative to wild-type-infected macrophages. The mutant exhibits significantly lower lung CFU during acute murine infection |
-, 761794 |
| 2.7.4.34 | physiological function |
PPK2 plays a role in mycobacterial survival under stress. A knockout mutant shows compromised survival under heat or acid stress or hypoxia, and the knockout is associated with reduction in GTP levels. PPK2 interacts with nucleoside diphosphate kinase (Ndk), the interaction requires G74 of PPK2 and 109LET111 of Ndk |
-, 761927 |
| 2.7.4.34 | physiological function |
PPK2 plays a role in mycobacterial survival under stress. A knockout mutant shows compromised survival under heat or acid stress or hypoxia. PPK2 interacts with nucleoside diphosphate kinase (Ndk), the interaction requires G74 of PPK2 and 109LET111 of Ndk |
-, 761927 |
| 2.7.4.34 | physiological function |
the deletion of Ppk2 results in a significant decrease in polyphosphate-dependent GTP synthesis, while displaying an increased intracellular ATP:GTP ratio. The Ppk2 deletion mutant exhibits a significant survival defect under osmotic, nutrient, aerobic, and antimicrobial stresses and displays an enhanced ability to form static biofilms. The mutant is not defective in polyphosphate and ppGpp synthesis. The Ppk2 mutant is significantly attenuated in invasion and intracellular survival within human intestinal epithelial cells as well as in chicken colonization |
-, 762249 |
