    2.6.1.85 | evolution |
common beans are excellent sources of dietary folates, but different varieties contain different amounts of these compounds. Dihydroneopterin aldolase (DHNA, EC 4.1.2.25) and aminodeoxychorismate synthase (ADCS) genes in the folate synthesis pathway, are characterized by PCR amplification, BAC clone sequencing, and whole genome sequencing. All DHNA and ADCS genes in the Mesoamerican cultivar OAC Rex are isolated and compared with those genes in the genome of Andean genotype G19833. Both genotypes have two functional DHNA genes and one pseudogene. There is only one ADCS gene (PvADCS) identified in the bean genome and it is identical in OAC Rex and G19833. PvADCS has the conserved motifs required for catalytic activity similar to other plant ADCS homologues. Genotype and geotype mapping |
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