   2.6.1.38 | more |
development and evaluation of a method for the prediction of possible genes of orphan enzyme reactions where any associated gene sequences are not determined to date. The candidate enzyme gene of a reaction is detected using the chemical structures of the substrate-product pair (reactant pair). The proposed method is based on a search for similar reactant pairs in a reference database (KEGG database) and offers orthologeous groups that possibly mediate the given reaction. One example is the histidine transaminase (R01161, EC 2.6.1.38), which transfers an amino group from histidine to glutamate. For the query consisting of histidine and imidazol-5-pyruvate (RP01339 in the RPAIR database), K00817 is predicted by the method with the highest score (0.971). The function of this KO entry is the transfer of an amino group from phenylalanine, tyrosine, and histidinol phosphate (histidinol-phosphate transaminase, EC 2.6.1.9), which are structurally similar to histidine, to glutamate. In addition, this KO entry contains the enzyme gene sequence tte:TTE2137 of Thermoanaerobacter tengcongensis, for which a histidine transaminase activity has been detected in the heterologous expression study |
-, 759546 |
