EC Number   |
General Information |
Reference |
|---|
   2.6.1.101 | evolution |
BtrR and StsC are the two homologues of Neo-6 in butirosin and streptamycin biosynthetic pathways |
-, 663004 |
| 2.6.1.101 | evolution |
BtrR has a fold characteristic of the aspartate aminotransferase family and is a member of the class of aminotransferases that function primarily in the biosynthesis of secondary metabolites, SMAT, i.e. the secondary metabolite aminotransferases subfamily |
723736 |
| 2.6.1.101 | metabolism |
purified Neo-6 also catalyses the amino transfer from L-glutamine to 2-deoxyscyllo-inosose to produce 2-deoxyscyllo-inosamine when coupled with BtrC in the presence of G6P, NAD+, Co2+ and L-glutamine, EC 2.6.1.100 |
-, 663004 |
| 2.6.1.101 | metabolism |
the aminotransferase BtrR is involved in the biosynthesis of butirosin |
723736 |
| 2.6.1.101 | metabolism |
the enzyme is involved in the biosynthesis of the 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. 3-Amino-2,3-dideoxy-scyllo-inosose produced by the NeoA reaction is continuously used as substrate for the natural forward transamination catalyzed by NeoB in the 2-deoxystreptamine biosynthesis. NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 |
-, 719670 |
| 2.6.1.101 | more |
active site structure of BtrR, homodimer with two active sites per dimer, BtrR with bound pyridoxamine 5'-phosphate lacks the covalent bond to Lys192. The active site of the BtrR protomer is located in a cleft between an alpha-helical N-terminus, a central alphabetaalpha-sandwich domain and an alphabeta-C-terminal domain |
723736 |
| 2.6.1.101 | more |
gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview |
-, 663004 |
| 2.6.1.101 | more |
the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS |
-, 719670 |
| 2.6.1.101 | physiological function |
the aminotransferase BtrR is involved in the biosynthesis of butirosin, a 2-deoxystreptamine-containing aminoglycoside antibiotic produced by Bacillus circulans, catalyzing the pyridoxal 5'-phosphate-dependent transamination reaction both of 2-deoxy-scylloinosose to 2-deoxy-scyllo-inosamine, cf. EC 2.6.1.100, and of amino-dideoxy-scyllo-inosose to 2-deoxystreptamine |
723736 |
| 2.6.1.101 | physiological function |
the enzyme produces 2-deoxystreptamine (DOS), which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis, function of Neo-6 as the L-glutamine:DOI aminotransferase |
-, 663004 |
