EC Number   |
General Information |
Reference |
|---|
   2.4.1.370 | malfunction |
deletion of the CSG2 gene reduces the Csg1p activity and abolishes the Csh1p activity. The DELTAcsg1/DELTAcsh1 double mutant is highly sensitive to Ca2+ and does not grow on the YPD plate medium |
-, 759459 |
| 2.4.1.370 | malfunction |
deletion of the CSG2 gene reduces the Csg1p activity and abolishes the Csh1p activity. The DELTAcsg2 mutant, as well as the DELTAcsg1/DELTAcsh1 double mutant, is highly sensitive to Ca2+ and does not grow on the YPD plate medium |
-, 759459 |
| 2.4.1.370 | malfunction |
DELTAcsg1 cells exhibit only a reduction in the synthesis of mannosylated sphingolipids compared with wild-type cells, whereas the DELTAcsg1/DELTAcsh1 double deletion mutant exhibits a total loss |
-, 759459 |
| 2.4.1.370 | malfunction |
the SUR1/CSG1 gene is a dose-dependent suppressor of the Ca2+-sensitive phenotype of the csg2 mutant, but overexpression of CSG2 does not suppress the Ca2+-sensitivity of the csg1 mutant. The csg1 and csg2 mutants display normal growth in YPD, indicating that mannosylation of sphingolipids is not essential. Increased osmolarity of the growth medium increases the Ca2+ tolerance of csg1 and csg2 mutant cells, suggesting that altered cell wall synthesis causes Ca2+-induced death. Phenotypic characterization of csg1 mutants, overview. Cu2+ reverses the Ca2+ sensitivity of csg1 mutant cells |
-, 759777 |
| 2.4.1.370 | metabolism |
sphingolipid metabolism and calcium sensitivity, overview |
-, 759777 |
| 2.4.1.370 | physiological function |
complex sphingolipids in yeast are known to function in cellular adaptation to environmental changes. One of the yeast complex sphingolipids, mannosylinositol phosphorylceramide (MIPC), is produced by the redundant inositol phosphorylceramide (IPC) mannosyltransferases Csg1 and Csh1. The Ca2+-binding protein Csg2 can form a complex with either Csg1 or Csh1 and is considered to act as a regulatory subunit. After complexing with Csg2, both Csg1 and Csh1 function in the Golgi, and then are delivered to the vacuole for degradation. Csg2 has several regulatory functions for Csg1 and Csh1, including stability, transport, and gene expression. Differing effects of Csg2 on the degradation of isozymes Csg1 and Csh1 in the vacuole |
-, 759463 |
| 2.4.1.370 | physiological function |
Csg1p and Csg2p are involved in the synthesis of mannosylinositol phosphorylceramide (MIPC) from inositol phosphorylceramide. Csg1p and Csh1p have redundant functions in MIPC synthesis. Csg2p interacts with both Csg1p and Csh1p. Two distinct inositol phosphorylceramide mannosyltransferase complexes, Csg1p-Csg2p and Csh1p-Csg2p, exist. Csg1p or Csh1p activity may be regulated by Ca2+ through Csg2p |
-, 759459 |
| 2.4.1.370 | physiological function |
Csh1p is involved in the synthesis of mannosylinositol phosphorylceramide (MIPC) from inositol phosphorylceramide. Csg1p and Csh1p have redundant functions in MIPC synthesis. Csg2p interacts with both Csg1p and Csh1p. Two distinct inositol phosphorylceramide mannosyltransferase complexes, Csg1p-Csg2p and Csh1p-Csg2p, exist. Csg1p or Csh1p activity may be regulated by Ca2+ through Csg2p |
-, 759459 |
| 2.4.1.370 | physiological function |
in the Golgi, IPC is mannosylated to form mannosylinositolphosphorylceramide (MIPC). Saccharomyces cerevisiae cells require two genes, CSG1/SUR1 and CSG2, for growth in 50 mM Ca2+, but not 50 mM Sr2+. CSG2 is previously shown to be required for the mannosylation of inositol-phosphorylceramide (IPC) to form mannosylinositol-phosphorylceramide (MIPC). SUR1/CSG1 is both genetically and biochemically related to CSG2. Like CSG2, SUR1/CSG1 is required for IPC mannosylation |
-, 759777 |
