EC Number   |
General Information |
Reference |
|---|
   2.4.1.203 | malfunction |
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview |
759270 |
| 2.4.1.203 | malfunction |
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview |
759270 |
| 2.4.1.203 | malfunction |
the accumulation level of the trans-zeatin O-glucosides is significantly increased in UGT85A1 overexpressing transgenic Arabidopsis thaliana, while other forms of cytokinins occur in concentrations similar to the wild-type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis thaliana plants show much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. The chlorophyll content of detached leaves of transgenic Arabidopsis thaliana plants is much lower than wild-type, phenotype, overview |
-, 723507 |
| 2.4.1.203 | malfunction |
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview |
759270 |
| 2.4.1.203 | metabolism |
in general, trans-zeatin (tZ) and N6-(DELTA2-isopentenyl)adenine (iP)-types predominate in the spectrum of cytokinins in Brassica napus, with N7-glucosides, namely tZ-N7-glucoside (tZ7G) and iPN7-glucoside (iP7G), representing the most abundant forms. Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves. Cytokinin spectrum and content, overview |
759242 |
| 2.4.1.203 | metabolism |
the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans |
-, 759242 |
| 2.4.1.203 | more |
homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates |
759043 |
| 2.4.1.203 | physiological function |
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol |
759270 |
| 2.4.1.203 | physiological function |
glucosyltransferase UGT85A1 is another zeatin O-glucosyltransferase with a preference for trans-zeatin. It influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation, regulation, overview |
-, 723507 |
