EC Number   |
General Information |
Reference |
|---|
   2.4.1.183 | evolution |
AgsE is one of five alpha-1,3-glucan synthase genes in Aspergillus luchuensis and a homologue of the major alpha-1,3-glucan synthase agsB in Aspergillus nidulans |
759525 |
| 2.4.1.183 | malfunction |
alpha-1,3-glucan is the main component of the alkali-soluble cell wall fraction in the wild-type and agsA disruption strains, but almost no alpha-1,3-glucan is found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Hyphal morphology of the control and agsB disruption strains, overview |
-, 737101 |
| 2.4.1.183 | malfunction |
deletion of all the three AGS genes results in a triple mutant that is devoid of alpha-(1,3)-glucan in its cell wall, buts growth and germination is identical to that of the parental strain in vitro. In the experimental murine aspergillosis model, this mutant is less pathogenic than the parental strain. The AGS deletion results in an extensive structural modification of the conidial cell wall, especially conidial surface where the rodlet layer is covered by an amorphous glycoprotein matrix. The surface modification is responsible for viability reduction of conidia in vivo, which explains decrease in the virulence of triple agsD mutant |
737151 |
| 2.4.1.183 | malfunction |
deletion of all the three AGS genes results in a triple mutant that is devoid of alpha-(1,3)-glucan in its cell wall, buts growth and germination is identical to that of the parental strain in vitro. In the experimental murine aspergillosis model, this mutant is less pathogenic than the parental strain. The AGS deletion results in an extensive structural modification of the conidial cell wall, especially conidial surface where the rodlet layer is covered by an amorphous glycoprotein matrix. Thie surface modification is responsible for viability reduction of conidia in vivo, which explains decrease in the virulence of triple agsD mutant |
737151 |
| 2.4.1.183 | malfunction |
disruption of agsE in Aspergillus luchuensis strain NBRC 4314 (DELTAagsE) shows that protoplast formation in DELTAagsE is comparable with protoplast formation in Aspergillus oryzae with commercial cell wall-digesting enzyme Yatalase. The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method |
759525 |
| 2.4.1.183 | malfunction |
disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain |
-, 758930 |
| 2.4.1.183 | malfunction |
disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain |
-, 758930 |
| 2.4.1.183 | malfunction |
overexpression of Aspergillus nidulans alpha-1,3-glucan synthase increases cellular adhesion and causes cell wall defects |
759714 |
| 2.4.1.183 | malfunction |
the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan |
-, 759245 |
| 2.4.1.183 | malfunction |
the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan |
-, 759245 |
