EC Number   |
General Information |
Reference |
|---|
  2.1.1.200 | evolution |
PA14 14690 possesses the conserved region of TrmJ-specific motif, a consensus TXARXR sequence (residues 79-84). This motif has been shown to be critical for binding of SAM/SAH and tRNA. PA14 14690 also contains a conserved Arg at position 23, which is proposed to be involved in its catalytic activity. Another catalytic residue, the Tyr at position 141, is replaced by Phe. In addition to Arg positions 82 and 84 in the TrmJ-specific motif, PA14 14690 also contains more key conserved tRNA binding residues including arginine at positions 100, 101 and 105 |
-, 757835 |
| 2.1.1.200 | evolution |
TrmJ proteins from the SPOUT methyltransferase superfamily are tRNA Xm32 modification enzymes that occur in bacteria and archaea. Unlike archaeal TrmJ, bacterial TrmJ require full-length tRNA molecules as substrates |
-, 736907 |
| 2.1.1.200 | malfunction |
loss of trmJ causes an increased susceptibility to H2O2. Phenotypic analysis of cytotoxicity induced by H2O2 in PA14 wild-type strain, trmJ mutant strain, and the trmJ complementation strain or trmJC, overview. Inactivation of trmJ abolishes Cm, Um, or Am formation in Pseudomonas aeruginosa tRNA |
-, 757835 |
| 2.1.1.200 | metabolism |
methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruginosa |
-, 757835 |
| 2.1.1.200 | more |
semi-quantitative real-time PCR analysis of oxyR, katA, katB, katE, ankB and recG transcripts in response to H2O2. Analysis of ribonucleosides by HPLC-coupled tandem quadrupole mass spectrometry |
-, 757835 |
| 2.1.1.200 | physiological function |
methylation of ribose moieties in tRNA is frequent, especially at position 32 where it is commonplace in all three domains of life |
-, 736907 |
| 2.1.1.200 | physiological function |
PA14 trmJ encodes a tRNA (cytidine(32)/uridine(32)/adenosine(32)-2'-O)-methyltransferase that regulates the expression of oxidative stress response genes. Role of tRNA modifications in the oxidative stress response of prokaryotes. TrmJ is a tRNA:Cm32/Um32/Am32 methyltransferase involved in translational fidelity and the oxidative stress response. TrmJ confers H2O2 resistance in part by upregulating the expression of catalase genes |
-, 757835 |
