EC Number   |
General Information |
Reference |
|---|
   2.1.1.142 | evolution |
due to differences in its overall amino acid composition and substrate-dependent partitioning pathways, Paracoccidiodes brasiliensis SMT has to be grouped into a fourth and new class of SMT |
-, 718966 |
| 2.1.1.142 | malfunction |
Deficiency of SMT2 in the cvp1 mutant results in moderate developmental defects, including aberrant cotyledon vein patterning, serrated floral organs, and reduced stature, but plants are viable, suggesting that SMT3 activity can substitute for the loss of SMT2. The overexpression of SMT3 complements the cotyledon venation pattern defects of cvp1 (SMT2) mutants |
720733 |
| 2.1.1.142 | malfunction |
smt3 single mutants appear wild type, cvp1 smt3 double mutants show enhanced defects relative to cvp1 mutants, such as discontinuous cotyledon vein pattern, and produce novel phenotypes, including defective root growth, loss of apical dominance, sterility, and homeotic floral transformations. Antisense smt3 plants did not display a phenotype, suggesting that SMT3 and SMT2 are functionally redundant |
720733 |
| 2.1.1.142 | malfunction |
the SMT2 genes of soybean (SMT2-1 and SMT2-2) are shown to complement the SMT deficient cvp1 mutant Arabidopsis plants |
720714 |
| 2.1.1.142 | malfunction |
transgenic Arabidopsis seeds expressing GmSMT1 or GmSMT2 show modifications in the ratio of 24-methyl to 24-ethyl sterol in the direction of sitosterol formation |
720714 |
| 2.1.1.142 | metabolism |
the enzyme is involved in the first metabolic step in the 24-alkyl sterol synthetic pathway of Paracoccidiodes brasiliensis involves the C24-methylation of lanosterol. PbSMT cannot perform the second alkylation step, consistent with the absence of sterols 24(28)-methylenelophenol, fecosterol or eburicol in wild-type cells. Alternate pathways and mechanisms for the conversion of ?24(25)-containing sterol side chains to methylated product, detailed overview |
-, 718966 |
| 2.1.1.142 | more |
residue Tyr88 in the native protein can contribute to stabilization of the active-site topography |
-, 718966 |
| 2.1.1.142 | physiological function |
enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions. Expression of SAM:SMT in a Saccharomyces cerevisiae Erg6 mutant restores its ability to produce ergosterol as the major sterol, and also results in low levels of C29 sterols such as pneumocysterol |
-, 745462 |
