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Results 1 - 8 of 8
EC Number General Information Commentary Reference
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function a strain carrying a deletion of the MET6 gene exhibits methionine as well as adenine auxotrophy. Methionine synthase targeted to the cytoplasm via a heterologous nuclear export signal, is expressed at very low levels and is unable to reverse methionine and adenine auxotrophy of a Met6 deletion strain 757179
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function deletion of MetE gene results in a rapid growth and glucose utilisation rate and almost loss of spinosad production. A total of 1440 genes are differentially expressed in wild-type and MetE mutant strains. The differentially expressed enzymes are mainly involved in primary metabolism and genetic information processing 733198
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function MetE is overexpressed in mutant cells lacking RNase E/G grown on various carbon sources. The level of MetE mRNA is also approximately 6- to 10fold higher in the RNase E/G mutant strain than in the wild-type strain. The half-life of MetE mRNA is approximately 4.2 times longer in the RNase E/G mutant than in the wild-type strain -, 757345
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function methionine synthase MS1 synthesizes the Met required for the activation of AtGLR3.5 Ca2+ channels whose expression is up-regulated during germination. Met synthesized via AtMS1 acts upstream of the AtGLR3.5-mediated Ca2+ signal and regulates the expression of ABI4, a major regulator in the abscisic acid response in seeds 757334
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function on minimum medium, MET6 null mutants are auxotrophic for methionine. Even when grown in presence of excess methionine, mutants display developmental defects, such as reduced mycelium pigmentation, aerial hypha formation and sporulation, and metabolic signatures such as increased levels of cysteine, cystathionine, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine while methionine and glutathione levels remain unchanged. the null mutant shows over-expression of CBS1 involved in the reversed transsulfuration pathway that metabolizes homocysteine into cysteine and SAM1 and SAHH1 involved in the methyl cycle. MET6 null mutants are non-pathogenic on both barley and rice leaves, and are defective in appressorium-mediated penetration and invasive infectious growth 735002
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function presence of B12-independent version of methionine synthase, METE, allows Fragilariopsis cylindrus to grow in the absence of B12 758312
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function substrate binding reorders the conformation of apo-Met6p to facilitate a direct SN2-type methylation reaction. The closed active site desolvates the active site save one water that serves as a conduit from His707 to protonate the tetrahydrofolate N5. Oscillations around the Zn cluster allow the thiolate to attack the methyl group and the protonated tetrahydrofolate is already activated as a reasonable leaving group. The transition state is likely to be a classic pyramidal structure with 3 methyl hydrogens in a plane and the incoming thiolate and departing tetrahydrofolate amine as axial ligands 757428
Show all pathways known for 2.1.1.14Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.14physiological function within L-methionine of natural origin, the S-methyl group is depleted in 13C by about 2% relative to the other positions in the molecule. Calculation predicts a strong normal 13C kinetic isotope effect (1.087) associated with methionine synthase. Depletion in 13C in the S-methyl of L-methionine during biosynthesis is an important factor contributing to the general depletion seen in many O-methyl and N-methyl groups of natural products 755895
Results 1 - 8 of 8