EC Number   |
General Information |
Reference |
|---|
   1.7.1.1 | evolution |
the enzyme contains a cysteine ligand and two oxido-ligands, and is a member of the sulfite oxidase, SO, family, defined by the identity of the ligands bound to the Mo center |
742026 |
| 1.7.1.1 | metabolism |
NarJ serves as a chaperone for both the anaerobic respiratory nitrate reductase (NarG, cf. EC 1.7.5.1) and the assimilatory nitrate reductase NasC, the latter of which is active during both aerobic and anaerobic nitrate assimilation. Both NasC and NarG are inactive in the absence of NarJ. 50% of NarJ binds in a 1:1 complex with NasC and the remaining 50% binds in a 1:1 complex with NarG |
-, 765398 |
| 1.7.1.1 | metabolism |
nitric oxide is an important signaling molecule regulating nitrate reductase activity, and cGMP participates as secondary messenger on this regulation by phosphorylation and desphosphorylation processes |
742767 |
| 1.7.1.1 | metabolism |
the reductive NO production pathway uses nitrite as substrate for NO production and involves either the nitrate reductase enzyme, the plasma membrane-bound nitrite:NO reductase, or mitochondrial nitrite reduction |
742026 |
| 1.7.1.1 | metabolism |
the reductive NO production pathway uses nitrite as substrate for NO production and involves either the nitrate reductase enzyme, the plasma membrane-bound nitrite:NO reductase, or mitochondrial nitrite reduction. Plant nitrate reductase-dependent mARC activity, NOFNiR, can catalyze NO production from nitrite in the presence of millimolar concentrations of nitrate, which strongly inhibits the NO producing nitrite reductase activity of the nitrate reductase. Two molybdoenzymes, nitrate reductase NR and nitrate reductase-dependent mARC, are needed together for NO production in plants. The dual system NR and mARC is the major player for NO production in plants. This cytosolic NO synthesis is strictly dependent on the nitrate reductase-diaphorase activity, and independent of the Moco domain of nitrate reductase |
742026 |
| 1.7.1.1 | physiological function |
a nitrate reductase-deficient mutant is capable of reducing nitrate at a rate sufficient to support growth rates approaching that of the control. The overall fate of the absorbed nitrate is basically similar between the two genotypes under light/dark cycle. Nitrate reduction in the mutant shoots is 9% lower than that in the control shoots at 38 h. Nitrate accumulation in mutant shoots is 78% higher than that in the control. Accumulation of reduced 15N in the mutant roots is 8% lower in the mutant shoots than in the control shoots at the end of the experiment |
765275 |
| 1.7.1.1 | physiological function |
a total of 74 genes are induced in cells grown with nitrate as N-source compared with ammonium, including nasTSABGHC and ntrBC genes. The nasABGHC genes constitute a transcriptional unit. The nasTS and nasABGHC transcripts are detected at similar levels with nitrate or glutamate as N-source, but nasABGHC transcript is undetectable in ammonium-grown cells. The nasT mutant lacks both nasABGHC transcript and nicotinamide adenine dinucleotide (NADH)-dependent nitrate reductase activity. The nasS mutant shows similar levels of the nasABGHC transcript to the wild-type strain and displays NasG protein and NADH-nitrate reductase activity with all N-sources tested, except with ammonium |
-, 764099 |
| 1.7.1.1 | physiological function |
isoform NIA1 is the more efficient nitrite reductase while isoform NIA2 exhibits higher nitrate reductase activity |
765636 |
| 1.7.1.1 | physiological function |
NarB, NarGHJI, dehydrogenase MSMEG_2237 and MSMEG_6816 are not required for nitrate reduction as MSMEG_4206 serves as the sole assimilatory nitrate reductase |
-, 765661 |
| 1.7.1.1 | physiological function |
nitrate reductase is the first enzyme in the nitrogen assimilatory pathway, which reduces nitrate to nitrite. Nitrate reductase is a key enzyme in nitrogen metabolism, that has been implicated in the production of nitric oxide (NO) in plants. In the red macroalga, nitrate reductase activity is modulated by photosynthetic electron transport chain and nitric oxide balance, direct dependence of nitrate reductase activity on the PSII and PSI electron flux, overview |
742767 |
