EC Number   |
General Information |
Reference |
|---|
   1.5.1.7 | malfunction |
single mutants in MDH3 or GPD1 grow on lysine-deficient medium, but an mdh3/gpd1DELTA double mutant accumulates saccharopine and displays lysine bradytrophy. Lysine biosynthesis is restored when saccharopine dehydrogenase is mislocalised to the cytosol in mdh3/gpd1DELTA cells. A decrease of saccharopine dehydrogenase activity (Lys1p-activity) causes lysine bradytrophy in mdh3/gpd1DELTA cells |
-, 765801 |
| 1.5.1.7 | metabolism |
in Saccharomyces cerevisiae, the ultimate step in lysine biosynthesis, the NAD+-dependent dehydrogenation of saccharopine to lysine, is a NAD+-dependent reaction performed inside peroxisomes. The availability of intraperoxisomal NAD+ required for saccharopine dehydrogenase activity can be sustained by both shuttles, the malate/oxaloacetate shuttle and a glycerol-3-phosphate dehydrogenase 1(Gpd1p)-dependent shuttle. The shuttles both are able to maintain the intraperoxisomal redox balance. The extent to which each of these shuttles contributes to the intraperoxisomal redox balance may depend on the growth medium. The presence of multiple peroxisomal redox shuttles allows eukaryotic cells to maintain the peroxisomal redox status under different metabolic conditions. During growth on glucose medium, saccharopine dehydrogenase (Lys1p) is the only lysine biosynthetic enzyme that is dependent on the availability of intraperoxisomal NAD+ |
-, 765801 |
| 1.5.1.7 | metabolism |
saccharopine dehydrogenase is the last enzyme in the alpha-aminoadipate pathway of L-lysine biosynthesis |
725754 |
| 1.5.1.7 | metabolism |
saccharopine dehydrogenase regulates the last step of fungal lysine biosynthesis |
-, 765014 |
| 1.5.1.7 | physiological function |
saccharopine dehydrogenase, encoded by the LYS1 gene, requires NAD+ for the production of lysine. Intraperoxisomal NAD+ is required for saccharopine dehydrogenase activity |
-, 765801 |
