EC Number |
General Information |
Reference |
---|
1.14.20.1 | evolution |
DAOCS is classified to the 2-oxoglutarate-Fe(II)-dioxygenase superfamily |
723902 |
1.14.20.1 | evolution |
the enzyme belongs to the iron- and 2-oxoglutarate-dependent oxidases |
-, 745495 |
1.14.20.1 | evolution |
the enzyme is a member of the 2-oxoglutarate oxygenase superfamily, the members have substantially conserved active sites and are proposed to employ a consensus mechanism proceeding via formation of an enzyme·Fe(II)-2OG-substrate ternary complex |
-, 744318 |
1.14.20.1 | metabolism |
the enzyme from Acremonium chrysogenum is bifunctional and catalyzes both the synthesis of deacetoxycephalosporin C from penicillin N, EC 1.14.20.1, as well as the hydroxylation of deacetoxycephalosporin C to deacetylcephalosporin C, EC 1.14.11.26. The activities are located on two different domains |
-, 725639 |
1.14.20.1 | more |
additional recombinant expression of gene cefF from Streptomyces clavuligerus in Acremonium chrysogenum strain CGMCC3.3795 leads to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis, overview |
-, 725639 |
1.14.20.1 | more |
determination of formation of a DAOCS·Fe(II)-2OG-penicillin N complex by nondenaturing ESI-MS analyses |
-, 744318 |
1.14.20.1 | more |
structure simulations, docking, and modeling, active site prediction, residues R162, F164, M180, L204, V245, V262, F264, and I305 are involved in 2-oxoglutarate binding, overview |
723902 |
1.14.20.1 | physiological function |
Cephalosporium acremonium expandase is a bifunctional enzyme, penicillin expandase, which catalyzes the ring expansion of penicillin N and the hydroxylation of deacetoxycephalosporin C (DAOC) to deacetylcephalosporin C (DAC). 2-oxoglutarate, Fe2+ and O2 are required for its catalytic activity. The enzyme is encoded by gene cefEF |
745495 |
1.14.20.1 | physiological function |
Cephalosporium acremonium expandase is a bifunctional enzyme, which catalyzes the ring expansion of penicillin N and the hydroxylation of deacetoxycephalosporin C (DAOC) to deacetylcephalosporin C (DAC). 2-oxoglutarate, Fe2+ and O2 are required for its catalytic activity. The enzyme is encoded by gene cefEF |
745495 |
1.14.20.1 | physiological function |
deacetoxycephalosporin C synthase (DAOCS) catalyzes the oxidative ring expansion of penicillin N (penN) to give deacetoxycephalosporin C (DAOC), which is the committed step in the biosynthesis of the clinically important cephalosporin antibiotics. The enzyme from Streptomyces clavuligerus shows an unusual ping-pong mechanism, which is significantly different from other members of the 2OG oxygenase superfamily |
-, 744318 |