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EC Number	General Information	Commentary	Reference
1.14.13.82	metabolism	lignin demethylation (O-demethylases) activity strips-off methyl groups in LMCs and KL and produces vicinal diols that covalently bind with Fe3+ to form Fe3+-catechol complexes	765236
1.14.13.82	metabolism	softwood lignin is a polymer composed of mainly guaiacyl (G) units, and thus the most reported aromatic compounds generated from pretreatment of softwood-based lignin are guaiacyl-derivates such as vanillin and guaiacol. Hardwood lignin, on the other hand, contains both guaiacyl (G) and syringyl (S) units and its depolymerization results, apart from the guaiacyl-derivates, in S-derivates such as syringaldehyde, syringate and syringol. Yet, only a few organisms are reported to metabolize compounds from syringyl-based (S) lignin. Microbacterium sp. RG1 can grow on syringate and vanillate	764310
1.14.13.82	metabolism	the enzyme is involved in the proposed catabolic pathways of lignin-derived phenols in Pseudomonas sp. strain NGC7 and engineered route for ccMA production. Gene pcaHG is essential for strain NGC7 to grow on vanillate, hydroxybenzoate (HBA), and protocatechuate (PCA), and the former two compounds are suggested to be catabolized via PCA while an aromatic ring cleavage enzyme gene different from pcaHG is involved in syringate catabolism	765192

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Release 2023.1 (January 2023)