   1.14.13.224 | physiological function |
flavin-dependent oxygenases, VioC and VioD, act sequentially in biosynthesis of violacein. VioD hydroxylates one indole ring at the 5-position to yield proviolacein, and VioC then acts on the other indole ring at the 2-position to create the oxindole and complete violacein formation. Deletion of both VioC and VioD leads to formation of a green pigment, deoxychromoviridans, in Top10 cells. When this same construct is transformed into BL21 cells, purple pigment is produced, containing deoxychromoviridans, prodeoxyviolacein, and various other two- and four-electron oxidized versions of prodeoxyviolacein |
-, 735666 |
