EC Number   |
General Information |
Reference |
|---|
    1.1.3.8 | malfunction |
a deficiency in GULO expression results in the inability to produce vitamin C. Using a previously derived Gulo-expressing vector, which produces murine GULO under the control of the murine cytomegalovirus (mCMV) promoter, a recombinant helper-dependent adenovirus (HDAd-mCMV-Gulo) is constructed that can be used to correct this genetic defect. A human liver cell line (Hep G2) infected with the HDAd-mCMV-Gulo vector expresses GULO in a time- and gene dose-dependent manner. These cells also produce ascorbic acid when exogenous gulonolactone is supplemented in the medium. Likewise, Gulo(-/-) mice treated with HDAd-mCMV-Gulo express GULO in the liver and produce ascorbic acid |
703948 |
| 1.1.3.8 | malfunction |
gulonolactone oxidase knockout mice that are unable to synthesize their own ascorbic acid during development |
711643 |
| 1.1.3.8 | malfunction |
L-gulono-gamma-lactone dehydrogenase knockout mice are unable to synthesize ascorbic acid (vitamin C) and are not protected from malaria infection |
711936 |
| 1.1.3.8 | metabolism |
L-gulono-gamma-lactone oxidase is a key enzyme for the biosynthesis of ascorbate, it catalyzes the final step of ascorbate biosynthesis in which L-gulonolactone is oxidized to ascorbate |
724785, 725053 |
| 1.1.3.8 | metabolism |
the enzyme catalyzes the last step of L-ascorbate biosynthesis |
742475 |
| 1.1.3.8 | more |
transgenic plants with increased vitamin C content in the fruit show enhanced tolerance to abiotic stresses induced by methyl viologen, NaCl, and mannitol as compared with wild-type plants. The leaf disc senescence assay show better tolerance in transgenic plants by retaining higher chlorophyll content compared with wild-type plants, phenotypes, overview |
725121 |
| 1.1.3.8 | physiological function |
GULO is a microsomal enzyme that catalyzes the aerobic conversion of gulonolactone to ascorbate, with the production of hydrogen peroxide. Desiccation induces Protopterus annectens to estivate, and six months of estivation lead to drastic decreases in gulo/Gulo expression and ascorbate concentration in the kidney. But high concentrations of ascorbate and ascorbate/dehydroascorbate are maintained in the brain during estivation, probably resulting from in situ ascorbate synthesis. The ability to synthesize ascorbate to ameliorate oxidative stress directly in the brain might contribute to the ability of the fish to undergo prolonged estivation on land. Ascorbate might act as an antistress agent since estivation can be a stressful event to the brain. For instance, ammonia excretion is impeded during the induction and maintenance phases of estivation due to a lack of water |
742475 |
| 1.1.3.8 | physiological function |
GULO is an old gene that predates the separation of Animals and Fungi. A GULO gene is present in the non-Bilateria Placozoa, Myxozoa and Anthozoa groups,and in Protostomians, in the Araneae family, the Gastropoda class, the Acari subclass, and the Priapulida, Annelida and Brachiopoda phyla lineages. Within Protostomes, GULO has been lost multiple times in large taxonomic groups, namely the Pancrustacea, Nematoda, Platyhelminthes and Bivalvia groups, a pattern similar to that reported for Vertebrate species |
762860 |
| 1.1.3.8 | physiological function |
L-gulono-gamma-lactone oxidase is a key enzyme for the biosynthesis of ascorbate, which is essential for several cellular functions. The fish vitamin C requirements are highest during early life stages |
724785 |
| 1.1.3.8 | physiological function |
L-gulono-gamma-lactone oxidase is a key enzyme required during the synthesis of L-ascorbic acid |
712049 |
