EC Number   |
General Information |
Reference |
|---|
   4.6.1.25 | more |
the recombinant protein RIII of T4 copurifies with the host ribosomal protein S1 in stoichiometric amounts |
772310 |
| 4.6.1.25 | physiological function |
inactivates a sub-class of early mRNA by cleaving Shine-Dalgamo sequences, and (ii) it is necessary for the degradation of early mRNAs, but not of middle and late mRNAs |
756744 |
| 4.6.1.25 | physiological function |
protein RIII is known as a cytoplasmic antiholin, which plays a role in the lysis inhibition function of T4. Protein RIII also acts as a viral effector protein mainly targeting Escherichia coli host S1 RNA-binding domains that are central for all the activities of this multifunctional protein. ribosomal protein S1 is the largest (61 kDa) flexible protein of the ribosome complex. The S1-RIII interaction prevents the S1-dependent activation of endoribonuclease RegB. During T4 phage infection, S1 acts as a cofactor of the T4 phage RegB endoribonuclease. S1 binds RNA substrates and promotes cleavage by RegB of the partially structured AG-rich RNAs and unstructured RNA having an 11-nucleotide-conserved sequence. The minimal domain combination required for stimulation of RegB nuclease is D4-D5, whereas the peptide containing all C-terminal domains (D3-D4-D5-D6) stimulates RegB to the same extent as the full-length protein. The activity of RNase RegB is limited to the early period of T4 infection, it is deactivated by protein RIII |
772310 |
| 4.6.1.25 | physiological function |
the enzyme controls the expression of a number of phage early genes |
754920 |
| 4.6.1.25 | physiological function |
the enzyme is necessary for the degradation of early but not middle or late mRNAs. The synthesis of several early proteins is down-regulated, probably as a consequence of RegB cleavages in the Shine-Dalgarno sequence of these genes. TRegB also regulates the translation of several prereplicative genes |
754415 |
| 4.6.1.25 | physiological function |
the enzyme participates in the bacteriophage T4 life cycle by favoring early messenger RNA breakdown |
754077 |
