EC Number   |
General Information |
Reference |
|---|
   3.1.21.4 | evolution |
the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems |
-, 714119 |
| 3.1.21.4 | malfunction |
treatment of pT7Blue plasmid with recombinant protein UPV229 completely blocks UpaP162 restriction enzyme activity |
-, 751983 |
| 3.1.21.4 | metabolism |
enzyme shows a random, sequential mechanism in which one double-stranded DNA at a time is cleaved within a fully-formed reaction synapse containing multiple bound DNA target sites |
774818 |
| 3.1.21.4 | metabolism |
measurement of restriction digest efficiency using digital cell-free protein synthesis. The quantitative measurements reveal a considerable variation in the digest efficiency among restriction endonucleases, from less than 70% to more than 99%. None of them shows truly complete digestion within reasonably long periods of reaction time |
-, 775340 |
| 3.1.21.4 | metabolism |
the balance between DNA methylation and cleavage of the type II restriction-modification system may be severely affected by transcriptional signals coming from outside the restriction-modification operon. By modulating the activity of the promoter, a broad range of restriction phenotypes for the EcoRI R-M system can be obtained that differ by up to 4 orders of magnitude in biological assays |
770699 |
| 3.1.21.4 | more |
comparison of the interatomic distances between metal ions and proposed key catalytic residues in the binding sites of seventeen type II restriction endonucleases, data taken from crystal structures |
714039 |
| 3.1.21.4 | more |
reaction mode of type IIB enzyme in one or two polypeptide systems, overview |
-, 714119 |
| 3.1.21.4 | more |
subunit BtsIB mutant shows a different digestion pattern from the wild type BtsI. The mutant BtsIB(R119A) acts as a different restriction enzyme with a previously unreported recognition sequence CAGTG(2/0), which is named as BtsI-1. Compared with wild-type BtsI, BtsI-1 shows different relative activities in NEB restriction enzyme reaction buffers NEB1, NEB2, NEB3 and NEB4 and less star activity. Similar to the wild-type BtsIB subunit, the BtsI-1 B subunit alone can act as a bottom nicking enzyme recognizing CAGTG(-/0) |
716676 |
| 3.1.21.4 | more |
type IIP restriction endonucleases are characterized by recognition sequences displaying dyad axes of symmetry (palindromes), and constitute the most abundant class of characterized restriction enzymes |
716374 |
| 3.1.21.4 | physiological function |
LmoJ2 protects against phages |
-, 729026 |
