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an alanine auxotroph (mutated in yfdZ) is isolated from a prototrophic double mutant deficient in AvtA and YfbQ, a novel alanine aminotransferase, by chemical mutagenesis; Escherichia coli synthesizes L-alanine by means of three aminotransferases, YfbQ, YfdZ, and AvtA; level of yfdZ expression in minimal medium is higher than in rich medium; an alanine auxotroph (mutated in yfdZ) is isolated from a prototrophic double mutant deficient in AvtA and YfbQ by chemical mutagenesis; Escherichia coli synthesizes L-alanine by means of three aminotransferases, YfbQ, YfdZ, and AvtA; YfbQ contributes to most of the alanine aminotransferase activity in rich medium
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