EC Number   |
General Information |
Reference  |
|---|
   2.1.1.202 | malfunction |
disruption of the ORF YBL024w leads to the complete absence of m5C in total yeast tRNA. No tRNA:m5C-methyltransferase activity towards all potential m5C methylation sites is detected in the extract of the disrupted yeast strain. The protein product of a single gene is responsible for complete m5C methylation of yeast tRNA |
639471 |
| 2.1.1.202 | physiological function |
the enzyme is responsible for complete m5C methylation of yeast tRNA |
639471 |
| 2.1.1.202 | metabolism |
formation of a covalent complex between dual-cysteine RNA:m5C methyltransferases and methylated RNA provides a unique means by which metabolic factors can influence RNA. By controlling the degree of formation of the enzyme-RNA covalent complex, S-adenosyl-L-homocysteine and pH are likely to influence the extent of m5C formation and the rate of release of methylated RNA from RNA:m5C methyltransferases. Metabolite-induced covalent complexes could plausibly affect the processing and function of m5C-containing RNAs |
735680 |
| 2.1.1.202 | more |
four active-site residues critical for Trm4p-mediated tRNA methylation are also required for the formation of the denaturant-resistant complexes with m5C-containing RNA |
735680 |
| 2.1.1.202 | evolution |
both the nucleotide position and percent methylation of tRNAs and rRNAs m5C sites are conserved across all species analysed |
735864 |
| 2.1.1.202 | evolution |
both the nucleotide position and percent methylation of tRNAs and rRNAs m5C sites are conserved across all species analysed, overview |
735864 |
| 2.1.1.202 | evolution |
both the nucleotide position and percent methylation of tRNAs and rRNAs m5C sites were conserved across all species analysed |
735864 |
| 2.1.1.202 | evolution |
Identification of m5C sites in nuclear, chloroplast and mitochondrial tRNAs. 39 cytosine 5-methylation sites are identified at 5 structural positions and are located in tRNA secondary structure at positions C38, C48, C49, C50 and C72, pattern of methylation in individual tRNA isodecoders, overview. Identification of single-nucleotide resolution of cytosine 5-methylation sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species. Both the nucleotide position and percent methylation of tRNAs and rRNAs cytosine 5-methylation sites are conserved across all species analysed, overview |
735864 |
| 2.1.1.202 | malfunction |
in trm4a defective mutants, the cytosine 5-methylation profile is the same as wild-type, showing that TRM4A is not required for methylation of any of the detected tRNAs. In contrast for trm4b-1 and trm4b-2 mutants, a total of 18 sites have no detectable methylation and 7 sites have reduced methylation when compared to wild-type, the sites are corresponding to structural positions C48, C49, and C50. trdmt1/trm4b double mutants are hypersensitive to the antibiotic hygromycin B |
735864 |
| 2.1.1.202 | physiological function |
post-transcriptional methylation of RNA cytosine residues to 5-methylcytosine (m5C) is an important modification that regulates RNA metabolism |
735864 |
