EC Number   |
General Information  |
Reference |
|---|
  2.1.1.182 | evolution |
the KsgA family belongs tothe group of S-adenosyl-L-methionine-dependent methyltransferases, known as class I MTases, KsgA is related to DNA adenosine methyltransferases, which transfer only a single methyl group to their target adenosine residue. Part of the discrimination between mono- and dimethyltransferase activity lies in a single residue in the active site, L114; this residue is part of a conserved motif, known as motif IV, which is common to a large group of S-adenosyl-L-methionine-dependent methyltransferases |
718932 |
| 2.1.1.182 | malfunction |
acquisition of high-level resistance to kasugamycin at an extraordinarily high frequency, ksgA mutants display a disadvantage in overall fitness compared to the parent strain |
701730 |
| 2.1.1.182 | malfunction |
cold sensitivity and altered ribosomal profiles are associated with a DELTAksgA genotype in Escherichia coli |
705774 |
| 2.1.1.182 | malfunction |
loss of this dimethylation confers resistance to the antibiotic kasugamycin |
-, 705141 |
| 2.1.1.182 | malfunction |
mutants which contain a frameshift mutation in ksgA are severely impaired for growth |
-, 702903 |
| 2.1.1.182 | malfunction |
spontaneous KSGR mutants in Neisseria gonorrhoeae arise through mutations in ksgA, which are likely to reduce KsgA activity and lead to undermethylated rRNA and thus resistance to kasugamycin |
704036 |
| 2.1.1.182 | malfunction |
strains lacking the methylase are resistant to kasugamycin |
705860 |
| 2.1.1.182 | malfunction |
the enzyme knockout strain is sensitive to oxidative stress and has a lower survival rate in murine macrophage RAW264.7 cells than the parent strain |
-, 733426 |
| 2.1.1.182 | malfunction |
the inability to form dimethyladenine in the 16S rRNA-derived oligonucleotide is accompanied by muatation from kasugamycin sensitivity to resistance |
705859 |
| 2.1.1.182 | malfunction |
yeast enzyme Dim1 complements heterologously for ksgA- mutation in Escherichia coli, demonstrating functional equivalence of the two proteins |
705108 |
