EC Number   |
General Information |
Reference |
|---|
   1.3.1.56 | metabolism |
NAD-dependent cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (BphB) catalyzes the second step of the PCB catabolic pathway in bacteria |
-, 745581 |
| 1.3.1.56 | evolution |
PbBphB is a member of the short-chain dehydrogenase/reductase (SRD) family |
-, 745581 |
| 1.3.1.56 | physiological function |
the enzyme is involved in the aerobic biodegradation of polychlorinated biphenyls |
728769 |
| 1.3.1.56 | physiological function |
the enzyme is the key enzyme in the biphenyl/polychlorinated biphenyl catabolic pathway |
-, 745581 |
| 1.3.1.56 | more |
the pocket between strand beta6 and helix alpha8 is the substrate-binding region and corresponds to a disordered region (Leu199-Ser206) in the structures of the apo and binary forms, dynamic behavior of the substrate binding loop. In the holo form, BPY stabilizes the conformation of the binding loop by interacting through the aromatic rings of reaction product 2,3-dihydroxybiphenyl with Val207 and Pro208 residues. Ile204 makes a hydrophobic interaction with the NAD+ cofactor only in the holo simulation. The lack of the latter interaction in the apo and in the binary forms explains the extended mobility of this region in the other two binding states of the protein |
-, 745581 |
