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Hfq-dependent small RNA MgrR negatively regulates expression of EptB. Envelope stress response protein Sigma E has positively regulates EptB. The effects of Sigma E and deletion of MgrR on levels of EptB mRNA are independent, and the same 5? end is found in both cases. Sigma E acts directly at the level of transcription initiation for EptB, from the same start point as Sigma 70. When Sigma E is active, synthesis of EptB transcript may outstrip MgrR-dependent degradation. A second sRNA, ArcZ, also directly and negatively regulates EptB
membranes from Escherichia coli grown on 5-50 mM CaCl2 contain a phosphoethanolamine transferase that uses the precursor Kdo2-[4*-32P]lipid IVA as an acceptor. Transferase is not present in membranes of Escherichia coli grown with 5 mM MgCl2, BaCl2, or ZnCl2
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