5.4.99.11 | Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose |
Glu295 plays a role as the general acid catalyst to protonate the oxygen of the glycosidic linkage for substrate hydrolysis, the Odelta2 of Asp241 acts as the nucleophile to attack the C1 of the D-glucosyl and forms a bond with C1 to obtain beta-glucosyl-enzyme intermediate, and the isomaltulose is produced when the O-6 of the hydrolysis product D-fructose nucleophilically binds to the C1 of the D-glucosyl group of the intermediate |
-, 746878 |