EC Number   |
Reaction |
Reference |
|---|
   4.3.1.1 | L-aspartate = fumarate + NH3 |
mechanism |
-, 5997, 6002, 6012, 6017, 6019, 6021, 6027, 649266, 651815, 651819, 652914 |
| 4.3.1.1 | L-aspartate = fumarate + NH3 |
mechanism, kinetics |
650750 |
| 4.3.1.1 | L-aspartate = fumarate + NH3 |
mechanism: overview |
649266 |
| 4.3.1.1 | L-aspartate = fumarate + NH3 |
reaction favors aspartate formation |
- |
| 4.3.1.1 | L-aspartate = fumarate + NH3 |
the catalytic mechanism is studied by using a combined quantum-mechanical/molecular-mechanical (QM/MM) approach. Two elementary steps are calculated: The first step is the abstraction of Cbeta proton of L-aspartate by Ser318, which is calculated to be rate limiting. The second step is the cleavage of Calpha- N bond of L-aspartate to form fumarate and ammonia. Ser318 functions as the catalytic base, whereas His188 is a dispensable residue, but its protonation state can influence the active site structure |
-, 730225 |
