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EC Number Reaction Commentary Reference
Show all pathways known for 4.2.1.150Display the reaction diagram Show all sequences 4.2.1.150a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain trans-2-enoyl-CoA + H2O - -
Show all pathways known for 4.2.1.150Display the reaction diagram Show all sequences 4.2.1.150a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain trans-2-enoyl-CoA + H2O the Mr-Crt catalytic mechanism constitutes a concerted attack by the two glutamate residues. While Glu143 of Mr-Crt protonates the substrate, Glu123 abstracts a proton from a bound water molecule. The Gly120 activates the substrate by a hydrogen bond to the oxygen of the enoyl moiety of the CoA ester. The binding pocket for the CoA moiety is formed by characteristic hydrophobic amino acids and lysine residues -, 748405
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