EC Number |
Reaction |
Reference |
---|
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
A blood coagulation factor formed from the proenzyme factor X by limited proteolysis. Factor X is a glycoprotein composed of a heavy chain and a light chain, which are generated from a precursor protein by the excision of the tripeptide RKR and held together by one or more disulfide bonds. The activated factor Xa converts prothrombin to thrombin in the presence of factor Va, Ca2+ and phospholipids. Scutelarin (EC 3.4.21.60) has similar specificity, but does not require factor Va. |
- |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
residues His57, Asp102, and Ser195 form the catalytic triad at the active site cleft between the two subdomains |
683309 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity |
649797, 649955, 649987, 650111, 650125, 650170, 650179, 650193, 650223, 650446, 650463, 650582, 650585, 650846, 650881, 650914, 650933, 650938, 650939, 651123, 651189, 651191, 652047, 652253, 652278, 652349, 652447, 652501, 652720, 652731, 652755, 653059, 653400, 653926, 653968, 653979, 653986 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity, autolysis loop is formed by residues Arg143, Lys147, Arg150, and Arg154 |
650164 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity, catalytic mechanism |
650336 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity, catalytic residues are Ser195 and His57, mechanism |
652728 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity, hydrolyzes two petide bonds in prothrombin having (Glu/Asp)-Gly-Arg-(Thr/Ile) as P3-P2-P1-P1' residues, glycine is not the best P2-residue, phenylalanine shows even higher activity at this position |
652262 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity, most selective P'2-position, less selective P'1-position, and non-selective P'3-position |
651531 |
3.4.21.6 | selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin |
scutelarin has similar specificity, Ser89 is critical for enzyme activity |
652682 |