EC Number   |
Reaction |
Reference |
|---|
    3.2.2.5 | NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ |
reaction mechanism, and structure-function relationship, overview |
709059 |
| 3.2.2.5 | NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ |
residues E146, D147, and W125 work collaboratively to facilitate the formation of the Michaelis complex |
683649 |
| 3.2.2.5 | NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ |
SPN shows an ordered uni-bi mechanism, with ADP-ribose being released as a second product. The catalytic mechanism requires an essential glutamic acid, Glu344, to stabilize an oxycarbenium ion intermediate, which subsequently is attacked by a nucleophile, a water molecule |
709150 |
| 3.2.2.5 | NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ |
the enzyme can also hydrolyse NADP+ to yield phospho-ADP-ribose and nicotinamide, but more slowly |
654609 |
| 3.2.2.5 | NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ |
the polar interactions between E226 and the substrate 2',3'-OH groups are essential for initiating catalysis. S193 has a regulatory role during catalysis and is likely to be involved in intermediate stabilization |
683631 |
| 3.2.2.5 | NAD+ + H2O = ADP-D-ribose + nicotinamide + H+ |
transglycosylation in mammalian and snake |
- |
