   3.2.1.63 | (Glcalpha(1-4))n + H2O = (Glcalpha(1-4))n-4 + (Glcalpha(1-4))4 |
stepwise reaction mechanism, the first step is the proton transfer from N423 to D766, and the second step involves the hydrolysis reaction via the inversion mechanism catalyzed by the amide group of N423. Assisted by D766, N423 serves as the general base to activate the water molecule to attack the anomeric carbon center. E566 is the general acid to facilitate the cleavage of glycosidic bond between L-fucose and galactose units. The intrinsic resonance structure for the side chain amide group of the asparagine residue is the origin to the catalytic activity |
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