EC Number |
Reaction |
Reference |
---|
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
activation mechanism |
652498 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
arrangement of two metal ions, a phosphate ion, and amino acid residues in the catalytic site is identical to that of PS/Tpases |
665427 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
comparison of kinetic properties of different transmembrane and cytosolic isozymes |
650430 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
conformation of active site is favorable for phosphotyrosine binding. Interaction of D115 and R13 to stabilize the conformation of the active cavity. A41 and G45 form a noncharged surface around the active cavity, which is surrounded by a flexible wall made of the loop region from F40 to T48 |
665402 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
for both alkyl and aryl substrates, including the activated substrate 4-nitrophenyl phosphate with its particularly good leaving group, general acid catalysis in PTPs is highly efficient and fully neutralizes the leaving group in the transition state. Mutating the conserved aspartic acid to glutamine causes the expected reductions in rate of several orders of magnitude and loss of the basic limb in pH-rate profiles, kinetic isotope effects, general acid catalysis mechanism, overview |
-, 749953 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
H148 acts as a general acid catalyst, D115 assists the protonation of the leaving group of a substrate, and D76 and D112 are involved in binding of magnesium ions |
664363 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
in addition to active site, enzyme has a low-affinity noncatalytic aryl phosphate-binding site |
664310 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism |
650928, 652000, 94997, 95002 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism of the dephosphorylation catalysed by MptpA |
714454 |
3.1.3.48 | [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate |
mechanism, features required for substrate |
652017 |