3.1.13.3 | exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates |
RNA binding and hydrolysis mechanism of oligoribonuclease CpsORN, overview. The His158 residue shows inward movement toward the active site to interact with the cleaved uridine phosphate, in the two-uridine complex structure. The side chain of His158 is also near the cleavage site of the U-U RNA substrate. The His158 is an activator of the water molecule during the enzymatic reaction of enzyme CpsORN. The manganese ion forms a new interaction with the 3'-oxygen of the ribose in the U1 ribonucleotide |
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