EC Number |
Reaction |
Reference |
---|
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
- |
- |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
esterase activity on chromophoric ester substrate, p-nitrophenyl acetate and the synthetic active-site titrant, trans-cinnamoyl imidazole |
94355 |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
mechanism |
94355, 94356, 94357, 94358 |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
similar catalytic triads are found in alpha/beta hydrolase fold enzymes: DLH is the simplest one |
94357, 94358, 94365 |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases |
94353, 94354, 94355, 94357, 94358, 94364 |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
the catalytic triad Cys123, His202 and Asp171 is a hybrid of the standard triads found in cysteine: Cys-His-Asn and serine: Ser-His-Asp proteases |
94356, 94358 |
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate |
wild-type enzyme catalytic triad: Cys-His-Asn, mutant enzyme catalytic triad: Ser-His-Asn |
-, 650835 |