EC Number |
Reaction |
Reference |
---|
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
5 amino acids, Lys15, Ser116, Asp35, Arg38, and Arg126, comprise the 5'-kinase active site, structure-activity relationships |
642028 |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
also acts on 5'-dephospho-RNA 3'-mononucleotides |
- |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
Asp38, as general base, activates the polynucleotide 5'-OH for its nucleophilic attack on the gamma phosphorus and Lys21 and Mg2+ stabilize the transition state, catalytic mechanism, overview |
723764 |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
mechanism involves a ternary complex of enzyme, ATP and DNA |
657957 |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
random order sequential mechanism |
642015 |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
random reaction mechanism |
642007 |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
reaction mechanism |
642024, 642030 |
2.7.1.78 | ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA |
structure of the active sites for 5'-polynucleotide kinase and 3'phosphatase activity of the bifunctional enzyme, substrate binding mechanism |
642030 |