EC Number   |
Reaction |
Reference |
|---|
    2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
acetylation of the synthase III-like polyketid synthase at Ser118 |
486934 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
active site cysteine |
486936, 486938, 486940, 486944, 486948 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
beta-ketoacyl-acyl-carrier protein synthase enzyme joins short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction, the reaction starts with a trans thioesterification of the acyl primer substrate from ACP to the enzyme, subsequently, the donor substrate malonyl-ACP is decarboxylated to form a carbanion intermediate, which in the third step attacks C1 of the primer substrate giving rise to an elongated acyl chain, H298 serves as a catalytic base in the decarboxylation step, the enzyme possesses a Cys-His-His catalytic triad, Lys328 has a dual role in catalysis: its charge influences acyl transfer to the active site Cys, and the steric restraint imposed on H333, as an obligate hydrogen bond donor at Ne, is of critical importance for decarboxylation activity, active sites of the wild-type KAS I, its H298 mutants, and their acyl complexes, overview |
673576 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
catalytic active site residues are Cys163 and His333, the active site contains two oxyanion holes, stabilizing the thioester oxyanion of acetylated CoA and of acetylated Cys92, respectively |
659726 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
comparison of active sites in isoforms KAS I, KAS II and related enzymes, geometry and catalytic role of equivalent residues |
660470 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
mechanism |
486863, 486864, 486944 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
mechanism and active site structure and function |
486943 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
mechanism, roles of H303, H337, C164 |
659029 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
structure |
486916 |
| 2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] |
structure of active site, substrate binding pocket, and subunit structure in relation to substrate specificity of the 3 isozyme types |
486940 |
