EC Number |
Reaction |
Reference |
---|
1.3.1.2 | 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ |
abstraction of pro-S hydrogen of NADPH and thus B-side stereospecific class dehydrogenase |
349220, 349223 |
1.3.1.2 | 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ |
comparison of substrate and cofactor binding to wild-type and mutant enzyme |
349226 |
1.3.1.2 | 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ |
mechanism, rate-determining half-reaction for reduction of flavin by NADPH has minor rate limitation, while the following protonation of flavin at N-1 is slow |
349235 |
1.3.1.2 | 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ |
nonclassical ping-pong mechanism |
349219 |
1.3.1.2 | 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ |
random rapid-equilibrium mechanism |
349234 |